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Western blot analysis of extracts of various cell lines, using IL2 antibody (16-547) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts of Raw264.7 cells, using IL2 antibody (16-547) at 1:1000 dilution. Raw264.7 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human tonsil using IL2 antibody (16-547) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using IL2 antibody (16-547) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IL2 antibody (16-547) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using IL2 antibody (16-547) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using IL2 antibody (16-547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

IL2 Antibody

CATALOG NUMBER: 16-547

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human IL2
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Raw264.7
Positive Control 2:
Jurkat
Positive Control 3:
A-549
Positive Control 4:
293T
Positive Control 5:
Mouse spleen
Positive Control 6:
Mouse testis
Predicted Molecular Weight:
Observed: 18kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IL2
Additional Names:
Interleukin 2Interleukin-2, T-cell growth factor, IL-2, TCGF, lymphokine
Ncbi Gene Id Number:
3558
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-547

List Size:
100 uL

List Price:

$436.00

Datasheet
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