Western blot analysis of extracts of various cell lines, using IL25 antibody (23-479) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat lung using IL25 antibody (23-479) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using IL25 antibody (23-479) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using IL25 antibody (23-479) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of human lung cancer cells using IL25 antibody (23-479) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using IL25 antibody (23-479) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 60s.

IL25 Antibody

CATALOG NUMBER: 23-479

Clonality:

Polyclonal

Tested Applications:

IF, IHC, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 33-177 of human IL25 (NP_073626.1).

Conjugate:

Unconjugated

Tested Applications:

IF, IHC, WB

Application Note:

WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:200

Positive Control 1:

U-937

Positive Control 2:

MCF7

Positive Control 3:

BT-474

Positive Control 4:

HepG2

Positive Control 5:

Mouse liver

Positive Control 6:

Mouse heart

Predicted Molecular Weight:

Observed: 24kDa

Advanced Validation

Properties

Purification:

Affinity purification

Clonality:

Polyclonal

Isotype:

IgG

Physical State:

Liquid

Buffer:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration:

batch dependent

Storage Conditions:

Store at -20°C. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

IL25

Additional Names:

IL25, interleukin 25, IL17E, interleukin 17E, interleukin-17E, interleukin-25

Ncbi Gene Id Number:

64806

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-479

List Size:

100 uL

List Price:

$436.00

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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IL25 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Positive Control 2:

Positive Control 3:

Positive Control 4:

Positive Control 5:

Positive Control 6:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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