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Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
IL25 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.

IL25 Antibody

CATALOG NUMBER: 57-895

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This IL25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-104 amino acids from the Central region of human IL25.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
20 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL25
Additional Names:
Interleukin-25, IL-25, Interleukin-17E, IL-17E, IL25, IL17E
Protein Accession Number:
Q9H293
Protein Gi Number:
20138730
Ncbi Gene Id Number:
64806
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a cytokine that sharessequence similarity with interleukin 17. This cytokine can induceNF-kappaB activation, and stimulate the production of interleukin8. Both this cytokine and interleukin 17B are ligands for thecytokine receptor IL17BR. Studies of a similar gene in mice suggestthat this cytokine may be a pro-inflammatory cytokine favoring theTh2-type immune response. Alternative splicing results in multipletranscript variants.
Background Reference 1:
Xu, G., et al. Allergy 65(5):581-589(2010)
Background Reference 2:
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Background Reference 3:
Goswami, S., et al. Nat. Immunol. 10(5):496-503(2009)
Background Reference 4:
Caruso, R., et al. Blood 113(15):3512-3519(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-895

List Size:
400 ul

List Price:

$495.00

Datasheet
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