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(LEFT)Western blot analysis of IL4 Antibody in WiDr cell line lysates (35ug/lane).IL4 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of IL4 Antibody in mouse cerebellum cell line lysates (35ug/lane).IL4(arrow) was detected using the purified Pab.
IL4 Antibody IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
(LEFT)Western blot analysis of IL4 Antibody in WiDr cell line lysates (35ug/lane).IL4 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of IL4 Antibody in mouse cerebellum cell line lysates (35ug/lane).IL4(arrow) was detected using the purified Pab.

IL4 Antibody

CATALOG NUMBER: 61-851

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This L4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-150 amino acids from the C-terminal region of human L4.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
17 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL4
Additional Names:
Interleukin-4, IL-4, B-cell stimulatory factor 1, BSF-1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, IL4
Protein Accession Number:
P05112
Protein Gi Number:
124337
Ncbi Gene Id Number:
3565
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome.
Background Reference 1:
Wong, H.L., et al. AIDS 24(7):1025-1033(2010) Davila, S., et al. Genes Immun. 11(3):232-238(2010) Trajkov, D., et al. IndianTuberc 56(3):117-131(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-851

List Size:
400 ul

List Price:

$495.00

Datasheet
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