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Flow cytometric analysis of Ramos cells using IL4R Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Western blot analysis of lysates from 293T, A431 cell line (from left to right), using IL4R Antibody at 1:1000 at each lane.
Flow cytometric analysis of Ramos cells using IL4R Antibody (green) compared to an isotype control of rabbit IgG(blue). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

IL4R Antibody

CATALOG NUMBER: 61-028

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This IL4R antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 677-691 amino acids from the C-terminal region of human IL4R.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For FACS starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
90 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL4R
Additional Names:
Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA, CD124, Soluble interleukin-4 receptor subunit alpha, Soluble IL-4 receptor subunit alpha, Soluble IL-4R-alpha, sIL4Ralpha/prot, IL-4-binding protein, IL4-BP, IL4R, IL4RA
Protein Accession Number:
P24394
Protein Gi Number:
124335
Ncbi Gene Id Number:
3566
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
Background Reference 1:
Idzerda R.L.,et al.J. Exp. Med. 171:861-873(1990).
Background Reference 2:
Galizzi J.-P.,et al.Int. Immunol. 2:669-675(1990).
Background Reference 3:
Kruse S.,et al.Int. Immunol. 11:1965-1970(1999).
Background Reference 4:
Loftus B.J.,et al.Genomics 60:295-308(1999).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-028

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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