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Western blot analysis of lysate from MDA-MB-453 cell line, using IL5RA Antibody at 1:1000 at each lane.
IL5RA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of lysate from MDA-MB-453 cell line, using IL5RA Antibody at 1:1000 at each lane.

IL5RA Antibody

CATALOG NUMBER: 61-848

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This IL5RA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-76 amino acids from the N-terminal region of human IL5RA.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
48 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL5RA
Additional Names:
Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125, CD125, IL5RA, IL5R
Protein Accession Number:
Q01344
Protein Gi Number:
116242525
Ncbi Gene Id Number:
3568
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IL5RA is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4.
Background Reference 1:
Matarin, M., et al. Stroke 40(11):3436-3442(2009)
Background Reference 2:
Beekman, J.M., et al. Blood 114(18):3917-3927(2009)
Background Reference 3:
Song, X.Y., et al. Diabetologia 52(8):1543-1553(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-848

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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