Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IL6 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: NCI-H460 whole cell lysate Lysates/proteins at 20 ug per lane.

IL6 Antibody

CATALOG NUMBER: 56-451

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This IL6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-55 amino acids from the Central region of human IL6.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
24 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IL6
Additional Names:
Interleukin-6, IL-6, B-cell stimulatory factor 2, BSF-2, CTL differentiation factor, CDF, Hybridoma growth factor, Interferon beta-2, IFN-beta-2, IL6, IFNB2
Protein Accession Number:
P05231
Protein Gi Number:
124347
Ncbi Gene Id Number:
3569
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a cytokine that functions ininflammation and the maturation of B cells. The protein isprimarily produced at sites of acute and chronic inflammation,where it is secreted into the serum and induces a transcriptionalinflammatory response through interleukin 6 receptor, alpha. Thefunctioning of this gene is implicated in a wide variety ofinflammation-associated disease states, including suspectibility todiabetes mellitus and systemic juvenile rheumatoid arthritis.
Background Reference 1:
Perez, A.B., et al. Hum. Immunol. 71(11):1135-1140(2010)
Background Reference 2:
Weng, X.M., et al. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 24(1):42-44(2010)
Background Reference 3:
Santhosh, S., et al. Trop Gastroenterol 31(1):30-33(2010)
Background Reference 4:
Gein, O.N., et al. Patol Fiziol Eksp Ter 1, 10-13 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-451

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369