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Western blot analysis in U251 cell line lysates (35ug/lane).
Western blot analysis in U251 cell line lysates (35ug/lane).

ILKAP Antibody

CATALOG NUMBER: 60-521

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This ILKAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-122 amino acids from the Central region of human ILKAP.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ILKAP
Additional Names:
Integrin-linked kinase-associated serine/threonine phosphatase 2C, ILKAP, ILKAP
Protein Accession Number:
Q9H0C8
Protein Gi Number:
74752560
Ncbi Gene Id Number:
80895
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a proteinserine/threonine phosphatase of the PP2C family. This protein caninteract with integrin-linked kinase (ILK/ILK1), a regulator ofintegrin mediated signaling, and regulate the kinase activity ofILK. Through the interaction with ILK, this protein may selectivelyaffect the signaling process of ILK-mediated glycogen synthasekinase 3 beta (GSK3beta), and thus participate in Wnt signalingpathway.
Background Reference 1:
Nakrieko, K.A., et al. Cell Cycle 7(14):2157-2166(2008)
Background Reference 2:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 3:
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Background Reference 4:
Hillier, L.W., et al. Nature 434(7034):724-731(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-521

List Size:
400 ul

List Price:

$495.00

Datasheet
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