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Western Blot in Hela cell line lysates (35ug/lane). IMP3 (arrow) was detected using the purified Pab.
IMP3 Antibody IHC analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot in Hela cell line lysates (35ug/lane). IMP3 (arrow) was detected using the purified Pab.

IMP3 Antibody

CATALOG NUMBER: 64-176

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This IMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the C-terminal region of human IMP3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IMP3
Additional Names:
U3 small nucleolar ribonucleoprotein protein IMP3, U3 snoRNP protein IMP3, BRMS2, IMP3, C15orf12, MRPS4
Protein Accession Number:
Q9NV31
Protein Gi Number:
52783099
Ncbi Gene Id Number:
55272
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the human homolog of the yeast Imp3 protein. The protein localizes to the nucleoli and interacts with the U3 snoRNP complex. The protein contains an S4 domain.
Background Reference 1:
Li, D., et al. Ann. Surg. Oncol. 16(12):3499-3506(2009) Walter, O., et al. Hum. Pathol. 40(11):1528-1533(2009) Riener, M.O., et al. Hum. Pathol. 40(10):1377-1383(2009) Noske, A., et al. Int. J. Gynecol. Pathol. 28(3):203-210(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-176

List Size:
400 ul

List Price:

$495.00

Datasheet
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