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Western blot analysis of extracts of various cell lines, using IMPA1 antibody (22-174) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using IMPA1 antibody (22-174) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using IMPA1 antibody (22-174) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using IMPA1 antibody (22-174) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using IMPA1 antibody (22-174). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using IMPA1 antibody (22-174) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

IMPA1 Antibody

CATALOG NUMBER: 22-174

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-277 of human IMPA1 (NP_005527.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
Positive Control 1:
SKOV3
Positive Control 2:
SW620
Positive Control 3:
Jurkat
Positive Control 4:
SH-SY5Y
Positive Control 5:
Mouse liver
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 30kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IMPA1
Additional Names:
IMP, IMPA, MRT59, inositol monophosphatase 1, D-galactose 1-phosphate phosphatase, IMP 1, IMPase 1, inositol(myo)-1(or 4)-monophosphatase 1, inositol-1(or 4)-monophosphatase 1, lithium-sensitive myo-inositol monophosphatase A1, myo-inositol monophosphatase 1, testicular tissue protein Li 94
Ncbi Gene Id Number:
3612
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an enzyme that dephosphorylates myo-inositol monophosphate to generate free myo-inositol, a precursor of phosphatidylinositol, and is therefore an important modulator of intracellular signal transduction via the production of the second messengers myoinositol 1,4,5-trisphosphate and diacylglycerol. This enzyme can also use myo-inositol-1,3-diphosphate, myo-inositol-1,4-diphosphate, scyllo-inositol-phosphate, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate, beta-glycerophosphate, and 2'-AMP as substrates. This enzyme shows magnesium-dependent phosphatase activity and is inhibited by therapeutic concentrations of lithium. Inhibition of inositol monophosphate hydroylosis and subsequent depletion of inositol for phosphatidylinositol synthesis may explain the anti-manic and anti-depressive effects of lithium administered to treat bipolar disorder. Alternative splicing results in multiple transcript variants encoding distinct isoforms. A pseudogene of this gene is also present on chromosome 8q21.13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-174

List Size:
100 uL

List Price:

$436.00

Datasheet
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