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45-771 (4ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Similar results were obtained after antigen retrieval at pH9.
45-771 (4ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Similar results were obtained after antigen retrieval at pH9.

Importin 8 Antibody

CATALOG NUMBER: 45-771

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SAFNFGTVPSNN
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Tonsil shows cytoplam / perinuclear staining in the parenchyma. Recommended concentration, 4-6ug/ml.
Specificity:
This antibody is expected to recognise isoform 1 (NP_006381.2) and isoform 2 (NP_001177924.1).
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
IPO8
Additional Names:
IPO8, importin 8, RANBP8, RAN-binding protein 8, FLJ26580, RAN binding protein 8
Protein Accession Number:
NP_006381.2,NP_001177924.1
Protein Gi Number:
53759103
Ncbi Gene Id Number:
10526
Background Reference 1:
Gorlich D, Dabrowski M, Bischoff FR, Kutay U, Bork P, Hartmann E, Prehn S, Izaurralde E. A novel class of RanGTP binding proteins. J Cell Biol. 1997 Jul 14;138(1):65-80.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-771

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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