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Western blot analysis of IN80B Antibody in K562 cell line lysates (35ug/lane)
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of IN80B Antibody in K562 cell line lysates (35ug/lane)

IN80B Antibody

CATALOG NUMBER: 61-680

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This IN80B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-174 amino acids from the Central region of human IN80B.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
39 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
INO80B
Additional Names:
INO80 complex subunit B, High mobility group AT-hook 1-like 4, IES2 homolog, hIes2, PAP-1-associated protein 1, PAPA-1, Zinc finger HIT domain-containing protein 4, INO80B, HMGA1L4, PAPA1, ZNHIT4
Protein Accession Number:
Q9C086
Protein Gi Number:
229462939
Ncbi Gene Id Number:
83444
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IN80B induces growth and cell cycle arrests at the G1 phase of the cell cycle.
Background Reference 1:
Miyako, K., et al. Mol. Endocrinol. 23(2):169-175(2009)
Background Reference 2:
Vertegaal, A.C., et al. Mol. Cell Proteomics 5(12):2298-2310(2006)
Background Reference 3:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-680

List Size:
400 ul

List Price:

$495.00

Datasheet
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