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Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 30ug HeLa lysate, Lane 2: 30ug HFF lysate, Lane 3: 30ug U2OS lysate).
Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in WB on Human cell lines at: 1:1000 (Lane 1: 30ug HeLa lysate, Lane 2: 30ug HFF lysate, Lane 3: 30ug U2OS lysate).

ING1 Antibody

CATALOG NUMBER: 30-622

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ING1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ING1 antibody can be used for detection of ING1 by ELISA at 1:62500. ING1 antibody can be used for detection of ING1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
32 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ING1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ING1
Additional Names:
ING1, p24ING1c, p33, p33ING1, p33ING1b, p47, p47ING1a
Protein Accession Number:
NP_937862
Protein Gi Number:
38201667
Ncbi Gene Id Number:
3621
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ING1 is a tumor suppressor protein that can induce cell growth arrest and apoptosis. The protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of ING1 gene have been detected in various cancers.This gene encodes a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53 signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.
Background Reference 1:
Ahmed, I.A., (2008) Colorectal Dis 10 (4), 344-351.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-622

List Size:
100 ul

List Price:

$473.00

Datasheet
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