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Immunohistochemical analysis of paraffin - embedded human lymphoid tissue showing cytoplasmic and membrane location using INHA antibody with DAB staining.
Immunohistochemical analysis of paraffin - embedded human ovary tumor tissue (top) and testicle tumor tissue (bottom) showing cytoplasmic and membrane location using INHA antibody with DAB staining.
Immunohistochemical analysis of paraffin - embedded human lymphoid tissue showing cytoplasmic and membrane location using INHA antibody with DAB staining.

INHA Antibody [4A2]

CATALOG NUMBER: 32-181

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant INHA expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:1,000
IHC(P):1:1,000 - 1:2,000
ELISA:Propose dilution 1:10,000
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
INHA(Inhibin alpha) monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
INHA
Additional Names:
Inhibin alpha chain
Protein Accession Number:
P05111
Protein Gi Number:
124274
Ncbi Gene Id Number:
3623
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
INHA(A-inhibin subunit precursor, inhibin alpha subunit ),also called inhibin(alpha) ,which is located on chromosome 2q33-q36. Inhibin is a gonadal protein that preferentially suppresses the secretion of pituitary follicle-stimulating hormone (FSH). Inhibin comprises of two subunits,Inhibin A and B. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumor suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa cell tumors and can therefore be used as a marker for primary as well as recurrent disease. In addition to their role in endocrine feedback in the reproductive sytem, inhibins subserve local regulatory roles in numerous extragonadal tissues, including brain, adrenal,bone marrow, placenta, and most notably anterior pituitary. Inhibin alpha subunit gene expression is down regulated in human prostate cancer, suggesting a tumor suppressive role.
Background Reference 1:
Mayo, K.E., Cerelli, G.M., Spiess, J., et al. 1986. Proc. Natl. Acad. Sci. USA 83: 5849-5853.
Background Reference 2:
GM LM, WF Crowley, Jr, AL Schneyer.1995.J. Clin. Endocrinol. Metab., Oct . 80:3043-3049.
Background Reference 3:
Knight, P.G. 1996.Front. Neuroendocrinol. 17: 476-509.
Background Reference 4:
PA Fahy, CA Wilson, AJ Beard, et al. 1995.J Endocrinol Nov.147:271-283.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-181

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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