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Western Blot in mouse testis tissue lysates (35ug/lane).NHA (arrow) was detected using the purified Pab.
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the INHA gene (Lane 2).
INHA Antibody IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of INHA Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot in mouse testis tissue lysates (35ug/lane).NHA (arrow) was detected using the purified Pab.

INHA Antibody

CATALOG NUMBER: 62-429

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This INHA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 79-108 amino acids from the N-terminal region of human INHA.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
40 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
INHA
Additional Names:
Inhibin alpha chain, INHA
Protein Accession Number:
P05111
Protein Gi Number:
124274
Ncbi Gene Id Number:
3623
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The inhibin alpha subunit joins either the beta A or beta B subunit to form a pituitary FSH secretion inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin have been shown to reflect the size of granulosa-cell tumors and can therefore be used as a marker for primary as well as recurrent disease. However, in prostate cancer, expression of the inhibin alpha-subunit gene was suppressed and was not detectable in poorly differentiated tumor cells. Furthermore, because expression in gonadal and various extragonadal tissues may vary severalfold in a tissue-specific fashion, it is proposed that inhibin may be both a growth/differentiation factor and a hormone.
Background Reference 1:
Balanathan,P., Br. J. Cancer 100 (11), 1784-1793 (2009)
Background Reference 2:
Mason,A.J., Mol. Endocrinol. 10 (9), 1055-1065 (1996)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-429

List Size:
400 ul

List Price:

$495.00

Datasheet
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