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Western blot analysis of extracts of mouse pancreas, using INS antibody (18-492) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat pancreatic islet using INS antibody (18-492) at dilution of 1:50 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreatic islet using INS antibody (18-492) at dilution of 1:50 (40x lens).
Immunofluorescence analysis of rat pancreas using INS antibody (18-492) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse pancreas using INS antibody (18-492) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of mouse pancreas, using INS antibody (18-492) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

INS Antibody

CATALOG NUMBER: 18-492

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human INS (NP_000198.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Mouse pancreas
Predicted Molecular Weight:
Observed: 12kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
INS
Additional Names:
INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin
Ncbi Gene Id Number:
3630
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-492

List Size:
100 uL

List Price:

$436.00

Datasheet
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