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Western blot analysis of INSC Antibody in WiDr cell line lysates (35ug/lane)
INSC Antibody IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of INSC Antibody in WiDr cell line lysates (35ug/lane)

INSC Antibody

CATALOG NUMBER: 64-073

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This INSC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 410-437 amino acids from the Central region of human INSC.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
63 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
INSC
Additional Names:
Protein inscuteable homolog, INSC
Protein Accession Number:
Q1MX18
Protein Gi Number:
116248172
Ncbi Gene Id Number:
387755
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
In Drosophila, neuroblasts divide asymmetrically into another neuroblast at the apical side and a smaller ganglion mother cell on the basal side. Cell polarization is precisely regulated by 2 apically localized multiprotein signaling complexes that are tethered by Inscuteable, which regulates their apical localization (Izaki et al., 2006 [PubMed 16458856]).
Background Reference 1:
Vural, A., et al. Mol. Cell. Biol. 30(6):1528-1540(2010)
Background Reference 2:
Rivadeneira, F., et al. Nat. Genet. 41(11):1199-1206(2009)
Background Reference 3:
Izaki, T., et al. Biochem. Biophys. Res. Commun. 341(4):1001-1006(2006)
Background Reference 4:
Katoh, M., et al. Int. J. Mol. Med. 11(1):111-116(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-073

List Size:
400 ul

List Price:

$495.00

Datasheet
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