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Antibody used in WB on Human Muscle at 0.2-1 ug/ml.
Antibody used in WB on Human 721_B at 1 ug/ml.
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.

INSIG1 Antibody

CATALOG NUMBER: 25-589

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human INSIG1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
INSIG1 antibody can be used for detection of INSIG1 by ELISA at 1:1562500. INSIG1 antibody can be used for detection of INSIG1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate
Predicted Molecular Weight:
25 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store INSIG1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
INSIG2
Additional Names:
INSIG1, MGC26273,
Protein Accession Number:
NP_057217
Protein Gi Number:
38327533
Ncbi Gene Id Number:
51141
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. INSIG1 is an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. INSIG1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins.The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Background Reference 1:
Li, C.G., (2008) Int. J. Cancer 123 (2), 273-282.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-589

List Size:
100 ul

List Price:

$519.00

Datasheet
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