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Western blot analysis of INSIG1 Antibody in Hela cell line lysates (35ug/lane)
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
INSIG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis of INSIG1 Antibody in Hela cell line lysates (35ug/lane)

INSIG1 Antibody

CATALOG NUMBER: 63-712

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This INSIG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-63 amino acids from the N-terminal region of human INSIG1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
30 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
INSIG1
Additional Names:
Insulin-induced gene 1 protein, INSIG-1, INSIG1
Protein Accession Number:
O15503
Ncbi Gene Id Number:
3638
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
INSIG1 is an insulin-induced protein. This protein encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins.
Background Reference 1:
Smith,E.M., et.al., J. Lipid Res. 51 (4), 701-708 (2010)
Background Reference 2:
Liu,X., et.al., Atherosclerosis 208 (2), 421-426 (2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-712

List Size:
400 ul

List Price:

$495.00

Datasheet
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