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IHC testing of human pancreas stained with Insulin antibody (2D11-H5). Note cytoplasmic staining of cells.
SDS-PAGE Analysis of Purified, BSA-Free Insulin Antibody (clone 2D11-H5 or INS05). Confirmation of Integrity and Purity of the Antibody.
IHC testing of human pancreas stained with Insulin antibody (2D11-H5). Note cytoplasmic staining of cells.

Insulin Antibody [2D11-H5 or INS05]

CATALOG NUMBER: 33-154

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Mouse
Species Reactivity:
Bovine, Human, Pig
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Bovine, Human, Pig
Immunogen:
Full length purified pig Insulin (aa 1-84) was used as the immunogen for this antibody.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for staining of formalin/paraffin tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Specificity:
Does not react with mouse and rat
Purification:
Protein G affinity chromatography
Clonality:
Monoclonal
Isotype:
IgG1, kappa
physical-state:
Liquid
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Storage Conditions:
Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Ncbi Official Symbol:
INS
Additional Names:
INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin
Ncbi Gene Id Number:
3630
User Note:
Optimal dilutions for each application to be determined by the researcher
Background:
This antibody recognizes a polypeptide which is identified as insulin, a 51-amino acid polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. antibody to insulin is an important beta-cell and insulinoma marker.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

33-154

List Size:
100 ug

List Price:

$626.00

Datasheet
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