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45-776 (0.1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-776 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-776 (2.5ug/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-776 (0.1ug/ml) staining of K562 cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Insulysin Antibody

CATALOG NUMBER: 45-776

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-QQYNFDRDNTE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110-120kDa band observed in lysates of cell line K562 (calculated MW of 118kDa according to NP_004960.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Lung and Breast. Recommended concentration: 2.5ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_004960.2; NP_001159418.1; NP_001309722.1; NP_001309723.1; NP_001309724.1; NP_001309726.1). Reported variants represent identical protein: NP_001309725.1, NP_001309724.1
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
IDE
Additional Names:
insulinase, insulysin, insulin-degrading enzyme , FLJ35968, INSULYSIN
Protein Accession Number:
NP_004960.2,NP_001159418.1,NP_001309722.1,NP_001309723.1,NP_001309724.1,NP_001309726.1
Protein Gi Number:
4826770
Ncbi Gene Id Number:
3416
Background Reference 1:
Zhao Z, Xiang Z, Haroutunian V, Buxbaum JD, Stetka B, Pasinetti GM. Insulin degrading enzyme activity selectively decreases in the hippocampal formation of cases at high risk to develop Alzheimer's disease. Neurobiol Aging. 2007 Jun;28(6):824-30. Epub 2006 Jun 12.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-776

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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