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Western blot analysis of INT10 Antibody in Hela cell line lysates (35ug/lane)
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of INT10 Antibody in Hela cell line lysates (35ug/lane)

INT10 Antibody

CATALOG NUMBER: 64-188

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This INT10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 516-545 amino acids from the C-terminal region of human INT10.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
82 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
INTS10
Additional Names:
Integrator complex subunit 10, Int10, INTS10, C8orf35
Protein Accession Number:
Q9NVR2
Protein Gi Number:
97052274
Ncbi Gene Id Number:
55174
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
INTS10 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit (POLR2A; MIM 180660) and mediates 3-prime end processing of small nuclear RNAs U1.
Background Reference 1:
Lasky-Su, J., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 147B (8), 1345-1354 (2008)
Background Reference 2:
Kooner, J.S., et al. Nat. Genet. 40(2):149-151(2008)
Background Reference 3:
Baillat, D., et al. Cell 123(2):265-276(2005)
Background Reference 4:
Yuryev, A., et al. Genomics 81(2):112-125(2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

64-188

List Size:
400 ul

List Price:

$495.00

Datasheet
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