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Western blot analysis of various lysates using Integrin alpha 1/CD49a Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20 s.
Confocal imaging of Neuro-2a cells using Integrin alpha 1/CD49a Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). The cells were counterstained with α-Tubulin Mouse mAb followed by incubation with PSFlo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of paraffin-embedded Mouse lung tissue using Integrin alpha 1/CD49a Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Western blot analysis of various lysates using Integrin alpha 1/CD49a Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20 s.

Integrin alpha 1/CD49a Rabbit Recombinant Antibody (83-305)

CATALOG NUMBER: 83-305

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P, IF/ICC, WB
Application Note:
WB,1:125000 - 1:500000, IF/ICC,1:200 - 1:800, IF-P,1:200 - 1:800, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
131kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Itga1
Additional Names:
Vla1; CD49A; E130012M19Rik
Protein Accession Number:
Q3V3R4
Ncbi Gene Id Number:
109700
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Predicted to enable collagen binding activity involved in cell-matrix adhesion; protein phosphatase binding activity; and signaling receptor binding activity. Involved in negative regulation of epidermal growth factor receptor signaling pathway. Acts upstream of or within cell adhesion; cellular extravasation; and neutrophil chemotaxis. Located in basal part of cell and external side of plasma membrane. Is expressed in blood vessel endothelium; embryo mesenchyme; intestine smooth muscle; and lung. Orthologous to human ITGA1 (integrin subunit alpha 1).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-305

List Size:
100 uL

List Price:

$477.00

Datasheet
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