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SDS-PAGE analysis of recombinant Integrin alpha 4 fragment on Coomassie Blue-stained 4-20% gradient gel.
SDS-PAGE analysis of recombinant Integrin alpha 4 fragment on Coomassie Blue-stained 4-20% gradient gel.

Integrin alpha 4 Recombinant Protein

CATALOG NUMBER: 95-110

Tested Applications:
ELISA, WB
Specifications
source-species:
E. coli
Species:
Human
source-species:
E. coli
Recombinant Protein Sequence:
aa 131 - 275
Fusion Tag:
Fusion Partner: N-terminal His-tag
Tested Applications:
ELISA, WB
Application Note:
This recombinant protein can be used for WB and ELISA. For research use only.
Predicted Molecular Weight:
17 kDa (Calculated)
Purity:
~95%
physical-state:
Liquid
Buffer:
50mM Tris pH7.5, 150mM NaCl, 5 mM MgCl2
Storage Conditions:
Store in working aliquots at -70°C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Ncbi Official Symbol:
ITGA4
Additional Names:
Integrin alpha 4 Antibody: IA4, CD49D, Integrin alpha-4, CD49 antigen-like family member D
Protein Accession Number:
NP_000876
Protein Gi Number:
67191027
Ncbi Gene Id Number:
3676
Background:
The integrin alpha 4 (also known as CD49d and ITGA4) belongs to the integrin alpha chain family of proteins. Integrins are heterodimeric integral membrane proteins composed of an alpha and beta chains (reviewed in 1). Alpha 4 (4) chain associates with either beta 1 (1) or beta 77) chain. It has been demonstrated that the putative ligand-binding sites of both integrin 41 and 47 is located on the 4 chain. These ligands included Madcam, VCAM, and fibronectin (2-4). Madcam is known as the principal ligand for integrin a4b7. Recently it was also demonstrated that HIV-1 envelope can mimic Madcam by binding to and signaling though integrin 47, the gut mucosal homing receptor for peripheral T cells (5). This recombinant protein fragment includes the ligand and monoclonal antibody binding domains; this binding has been tested and verified in ELISA assays. This recombinant protein is recognized PSI antibodies Integrin 4 (IN1) (PSI 4827) and Integrin 4 (IN2) (PSI 4837), but not Integrin 4 (CT) (PSI 4783).
Background Reference 1:
Wegener KL and Campbell ID. Transmembrane and cytoplasmic domains in integrin activation and protein-protein interactions. Mol. Membr. Biol. 2008; 25:376-87.
Background Reference 2:
Kamata T, Puzon W, and Takada Y. Identification of putative ligand-binding sites of the integrin alpha 4 beta 1 (VLA-4, CD49d/CD29). Biochem J. 1995; 305:945-51.
Background Reference 3:
Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 1993; 74:185-95.
Background Reference 4:
Pulido R, Elices MJ, Campanero MR, et al. Functional evidence for three distinct and independently inhibitable adhesion activities mediated by the human integrin VLA-4. Correlation with distinct alpha 4 epitopes. J. Biol. Chem. 1991; 266:10241-5.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

95-110

List Size:
0.05 mg

List Price:

$289.00

Datasheet
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