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Western blot analysis of lysates from A-549 cells, using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 1s.
Western blot analysis of various lysates using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.
Western blot analysis of various lysates using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Flow cytometry:1X10^6 Daudi cells (negative control,left) and K-562 cells (right) were intracellularly-stained with Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb or Rabbit IgG isotype control,followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 Daudi cells (negative control,left) and U-87MG cells (right) were intracellularly-stained with Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb or Rabbit IgG isotype control,followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb(1:600 dilution) staining. Non-fluorescently stained cells were used as blank control (red line).
Western blot analysis of lysates from A-549 cells, using Integrin alpha 5 (ITGA5/CD49e) Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 1s.

Integrin alpha 5 (ITGA5/CD49e) Rabbit Recombinant Antibody (81-347)

CATALOG NUMBER: 81-347

Clonality:
Monoclonal
Tested Applications:
ELISA, FC (intra), IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC (intra), IHC-P, WB
Application Note:
WB,1:1000 - 1:6000, IHC-P,1:200 - 1:2000, FC (intra),1:50 - 1:200, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
HeLa, A-549, NIH/3T3, U-937, Mouse liver, Mouse heart, Mouse lung, Mouse large intestine, Mouse spleen, Rat lung, Rat liver, Rat heart
Predicted Molecular Weight:
115kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ITGA5
Additional Names:
FNRA; CD49e; VLA-5; VLA5A; Integrin alpha 5 (ITGA5/CD49e)
Protein Accession Number:
P08648
Ncbi Gene Id Number:
3678
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor. This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-347

List Size:
100 uL

List Price:

$540.00

Datasheet
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