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Western blot analysis of extracts of various cell lines, using ITGB1 antibody (13-899) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse stomach using ITGB1 antibody (13-899) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using ITGB1 antibody (13-899) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using ITGB1 antibody (13-899) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ITGB1 antibody (13-899) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using ITGB1 antibody (13-899) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using ITGB1 antibody (13-899) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse colon using ITGB1 antibody (13-899) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using ITGB1 antibody (13-899) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

Integrin beta 1 Antibody [F3F7]

CATALOG NUMBER: 13-899

Clonality:
Monoclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human ITGB1
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HepG2
Positive Control 2:
HeLa
Positive Control 3:
U-87MG
Positive Control 4:
HT-1080
Positive Control 5:
Mouse kidney
Predicted Molecular Weight:
Observed: 100-130kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ITGB1
Additional Names:
ITGB1, integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12), CD29, FNRB, GPIIA, MDF2, MSK12, VLAB, OTTHUMP00000046253, OTTHUMP00000063731, OTTHUMP00000063732, OTTHUMP00000063733, fibronectin receptor beta subunit, i
Ncbi Gene Id Number:
3688
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-899

List Size:
50 uL

List Price:

$365.00

Out of stock

Datasheet
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