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Western blot of immunoprecipitates from HEK 293 cells transfected with: 1. Mock 2. IFNAR1 WT and 3. IFNAR1 Ser535Arg and Ser 539Art mutants. The immunolabeling is absent in the IFNAR1 Ser535 and Ser539 mutants. The labeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the dephosphopeptide (Dephos).
Western blot of immunoprecipitates from HEK 293 cells transfected with: 1. Mock 2. IFNAR1 WT and 3. IFNAR1 Ser535Arg and Ser 539Art mutants. The immunolabeling is absent in the IFNAR1 Ser535 and Ser539 mutants. The labeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the dephosphopeptide (Dephos).

Interferon-a Receptor (phospho Ser535 / 539) Antibody

CATALOG NUMBER: XPS-1014

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Bovine, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Bovine, Human, Mouse, Rat
Immunogen:
IFNAR1 (Ser535/539) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acid residues surrounding the human Phospho-Ser535, 539 Type I Interferon-a Receptor Subunit 1 (IFNAR1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificity:
Interferon a Receptor antibody is specific for IFNAR1 phosphorylated at Ser535,539.
Predicted Molecular Weight:
110-130
Purification:
Affinity Purified
Clonality:
Polyclonal
physical-state:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage –80°C is recommended, but shorter term storage at –20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Ncbi Official Symbol:
IFNAR1
Protein Accession Number:
P17181
Protein Gi Number:
90110827
Ncbi Gene Id Number:
3454
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system. These cytokines produce their effects by binding to the Type 1 Interferon-alpha Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1.
Background Reference 1:
Suresh Kumar, K.G., Tang, W., Ravindranath, A.K., Clark, W.A., Croze, E., Fuchs, S.Y., "SCFHOS ubiquitin ligase mediates the ligand-induced down-regulation of the interferon- receptor," The EMBO Journal, 22 (2003), 5480-5490.
Background Reference 2:
Kirkwood, J., "Cancer immunotherapy: the interferon- experience," Semin. Oncol., 29 (2002) 18-26.
Background Reference 3:
Biron, C.A., "Interferons alpha and beta as immune regulators-a new look," Immunity, 14 (2001) 661-664.
Background Reference 4:
Stark, G.R., Kerr, I.M., Williams, B.R., Silverman, R.H. and Schreiber, R.D. "How cells respond to interferons." Annu. Rev. Biochem., 67 (1998) 227-264.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1014

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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