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Western blot analysis of extracts of various cell lines, using INTS6 antibody (22-368) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human breast cancer using INTS6 Antibody (22-368) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using INTS6 Antibody (22-368) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using INTS6 antibody (22-368). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using INTS6 antibody (22-368) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

INTS6 Antibody

CATALOG NUMBER: 22-368

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human INTS6 (NP_036273.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HL-60
Positive Control 2:
SW620
Positive Control 3:
HeLa
Positive Control 4:
NCI-H460
Positive Control 5:
U-251MG
Predicted Molecular Weight:
Observed: 115kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
INTS6
Additional Names:
DBI-1, DDX26, DDX26A, DICE1, HDB, INT6, Notchl2, integrator complex subunit 6, DEAD box protein, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 26, RNA helicase HDB, protein deleted in cancer 1
Ncbi Gene Id Number:
26512
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. The protein encoded by this gene is a DEAD box protein that is part of a complex that interacts with the C-terminus of RNA polymerase II and is involved in 3' end processing of snRNAs. In addition, this gene is a candidate tumor suppressor and is located in the critical region of loss of heterozygosity (LOH). Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-368

List Size:
100 uL

List Price:

$436.00

Datasheet
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