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To a sandwich ELISA (assuming 100 ul/well), a concentration of 1.0-2.0 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IP-10 when used with ProSci’s Biotinylated Antigen Affinity Purified anti-Human IP-10 (XP-5203Bt) as the detection antibody at a concentration of approximately 0.25 ug/ml.
To detect Human IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml (non-reducing) or at a concentration of 1.0-2.0 ug/ml (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.
To detect Human IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml (non-reducing) or at a concentration of 1.0-2.0 ug/ml (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.
To a sandwich ELISA (assuming 100 ul/well), a concentration of 1.0-2.0 ug/ml of this antibody will detect at least 1000 pg/ml of recombinant Human IP-10 when used with ProSci’s Biotinylated Antigen Affinity Purified anti-Human IP-10 (XP-5203Bt) as the detection antibody at a concentration of approximately 0.25 ug/ml.

IP-10 Antibody [3.3-1G1-2G7-2F6]

CATALOG NUMBER: 38-267

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Produced in BALB/c mice using highly pure recombinant Human IP-10 as the immunizing antigen. This antibody was purified from cell culture by Protein A affinity chromatography.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 1000 pg/mL of recombinant Human IP-10 when used with ProSci’s Biotinylated Antigen Affinity Purified anti-Human IP-10 (38-252) as the detection antibody at a concentration of approximately 0.25 μg/mL.
Western Blot:
To detect Human IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL (non-reducing) or at a concentration of 1.0-2.0 μg/mL (reducing). When used in conjunction with compatible secondary reagents the detection limit for recombinant Human IP-10 is 0.20-0.40 ng/lane under non-reducing conditions and 2.00-4.00 ng/lane under reducing conditions.
Clonality:
Monoclonal
Physical State:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CXCL10
Additional Names:
CXCL10, INP10, SCYB10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Gamma-IP10, C7, IFI10, IP-10, crg-2, mob-1, gIP-10
Protein Accession Number:
P02778
Protein Gi Number:
21542456
Ncbi Gene Id Number:
3627
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-267

List Size:
0.5 mg

List Price:

$376.00

Datasheet
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