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To detect Rat IP-10 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat IP-10 (38-255) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IP-10.
To detect Rat IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Rat IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained colchicine injected rat brain (including the caudate putamen) tissue. The primary antibody was incubated at 0.25 ug/ml overnight at 4C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
To detect Rat IP-10 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat IP-10 (38-255) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IP-10.

IP-10 Antibody

CATALOG NUMBER: 38-252

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Rabbit
Species Reactivity:
Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Rat
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Human Heregulin- β1. Human Heregulin- β1 specific antibody was purified by affinity chromatography and then biotinylated.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IP-10 (100 ng/mL), a concentration of 5.0-10.0 μg/mL of this antibody is required.
Sandwich
To detect Rat IP-10 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat IP-10 (38-255) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IP-10.
Western Blot:
To detect Rat IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
The lyophilized antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-80°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Cxcl10
Additional Names:
IP-10, Scyb10, Inp10, Mob1, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Gamma-IP10
Protein Accession Number:
P48973
Protein Gi Number:
146345511
Ncbi Gene Id Number:
245920
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-252

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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