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Western blot analysis of IPR1 in Hela cell lysate with IPR1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunohistochemical staining of human brain tissue using IPR1 antibody at 2.5 μg/mL.
Immunofluorescence of IPR1 in Human Brain cells with IPR1 antibody at 50 μg/mL.
Western blot analysis of IPR1 in Hela cell lysate with IPR1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Trial Size

IPR1 Antibody

CATALOG NUMBER: 4407

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IPR1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human IPR1.
The immunogen is located within amino acids 470 - 520 of IPR1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Ipr1 antibody can be used for detection of Ipr1 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
IPR1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IPR1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IPR1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SP110
Additional Names:
IPR1 Antibody: IPR1, VODI, IFI41, IFI75, Sp110 nuclear body protein, Speckled 110 kDa
Protein Accession Number:
NP_004501
Protein Gi Number:
190343008
Ncbi Gene Id Number:
3431
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IPR1 Antibody: Susceptibility to tuberculosis (TB) in mice has recently been attributed to the IPR1 gene. IPR1 is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. Alternative splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified. SP110 is the closest homolog of the IPR1 protein in humans. The IPR1/Sp110 gene product might play a role in integrating signals generated by intracellular pathogens with mechanisms controlling innate immunity, cell death, and pathogenesis. IPR1/Sp110 is up-regulated after infection with M. tuberculosis and required by Anaplasma phagocytophilum for infection of human promyelocytic cells. Defects in Sp110 are a cause of severely impaired resistance to infection by M. tuberculosis.
Background Reference 1:
Pan H, Yan BS, Rojas M, et al. Ipr1 gene mediates innate immunity to tuberculosis. Nature2005; 434:767-72.
Background Reference 2:
Bloch DB, Nakajima A, Gulick T, et al. Sp110 localizes to the PML-Sp100 nuclear body and may function as a nuclear hormone receptor transcriptional coactivator. Mol. Cell Biol.2000; 20:6138-46.
Background Reference 3:
De la Fuente J, Manzano-Roman R, Blouin EF, et al. Sp110 transcription is induced and required by Anaplasma phagocytophilum for infection of human promyelocytic cells. BMC Infect. Dis.2007; 7:110.
Background Reference 4:
Tosh K. Campbell SJ, Fielding K, et al. Variants in the SP110 gene are associated with genetic susceptibility to tuberculosis in West Africa. Proc. Natl. Acad. Sci.2006; 103:10364-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4407

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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