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Western blot analysis in T47D cell line lysates (35ug/lane).
Western blot analysis in T47D cell line lysates (35ug/lane).

IQGAP1 Antibody

CATALOG NUMBER: 58-784

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This IQGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-285 amino acids from the N-terminal region of human IQGAP1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
189 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IQGAP1
Additional Names:
Ras GTPase-activating-like protein IQGAP1, p195, IQGAP1, KIAA0051
Protein Accession Number:
P46940
Protein Gi Number:
1170586
Ncbi Gene Id Number:
8826
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the IQGAP family. Theprotein contains four IQ domains, one calponin homology domain, oneRas-GAP domain and one WW domain. It interacts with components ofthe cytoskeleton, with cell adhesion molecules, and with severalsignaling molecules to regulate cell morphology and motility.Expression of the protein is upregulated by gene amplification intwo gastric cancer cell lines.
Background Reference 1:
Hayashi, H., et al. Int. J. Cancer 126(11):2563-2574(2010)
Background Reference 2:
Liang, S., et al. Proteomics 10(7):1474-1483(2010)
Background Reference 3:
Nakhaei-Nejad, M., et al. Eur. J. Immunol. 40(1):204-213(2010)
Background Reference 4:
Wang, J.B., et al. J. Cell. Sci. 122 (PT 12), 2024-2033 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-784

List Size:
400 ul

List Price:

$495.00

Datasheet
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