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Western blot analysis of IRAK in mouse liver lysate with IRAK antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of IRAK in rat liver tissue with IRAK antibody at 5 μg/mL.
Western blot analysis of IRAK in mouse liver lysate with IRAK antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

IRAK Antibody [8F1A7]

CATALOG NUMBER: PM-5317

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein corresponding to amino acids 618 - 712 of human IRAK.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
IRAK antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 95-112 - IRAK Recombinant Protein
Purification:
IRAK Monoclonal Antibody is Protein G purified.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
IRAK Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IRAK monoclonal antibody can be stored at -20°C, stable for one year.
Ncbi Official Symbol:
IRAK1
Additional Names:
IRAK Antibody [8F1A7] : IRAK, pelle
Protein Accession Number:
P51617
Protein Gi Number:
8928535
Ncbi Gene Id Number:
3654
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRAK Monoclonal Antibody: Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-κB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).
Background Reference 1:
Cao Z,; Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31.
Background Reference 2:
Trofimova M, Sprenkle AB, Green M; et al. Developmental and tissue-specific expression of mouse pelle-like protein kinase. J. Bio. Chem. 1996; 271: 17609-12.
Background Reference 3:
Jianing Huang, Xiong Gao, Shyun Li, et al. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin-1 receptor accessory protein. Proc. Natl. Acad. Sci. USA 1997; 94:12829-32.
Background Reference 4:
Robinson D, Shibuya K, Mui A, et al. IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NF-kB. Immunity 1997; 7:571-81.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

PM-5317

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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