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Western blot analysis of IRAK-M in (M) mouse spleen and (R) rat liver tissue lysates with IRAK-M antibody at 1 μg/mL.
Immunohistochemistry of IRAK-M in human spleen tissue with IRAK-M antibody at 5 μg/mL.
Immunofluorescence of IRAK-M in Rat Liver tissue with IRAK-M antibody at 10 μg/mL.
Immunohistochemical staining of rat liver tissue using IRAK-M antibody at 2 μg/mL.
Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 20 μg/mL.<br><br>Red: IRAK-M Antibody (2355) <br> Blue: DAPI staining
Western blot analysis of IRAK-M in (M) mouse spleen and (R) rat liver tissue lysates with IRAK-M antibody at 1 μg/mL.
Trial Size

IRAK-M Antibody

CATALOG NUMBER: 2355

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IRAK-M antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRAK-M.
The immunogen is located within the last 50 amino acids of IRAK-M.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
IRAK-M antibody can be used for detection of IRAK-M by Western blot at 1 μg/mL. IRAK-M antibody can also detect IRAK-M by immunohistochemistry at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in human and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Specificity:
IRAK-M antibody is predicted to have no cross reactivity to other members of the IRAK family.
Positive Control 1:
Cat. No. 1406 - Mouse Spleen Tissue Lysate
Positive Control 2:
Cat. No. 1465 - Rat Kidney Tissue Lysate
Positive Control 3:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 66 kDa
Observed: 70 kDa
Purification:
IRAK-M Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IRAK-M Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IRAK-M antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IRAK3
Additional Names:
IRAK-M Antibody: ASRT5, IRAKM, Interleukin-1 receptor-associated kinase 3, IL-1 receptor-associated kinase M, IRAK-3
Protein Accession Number:
NP_009130
Protein Gi Number:
5225377
Ncbi Gene Id Number:
11213
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRAK-M Antibody: Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-betaB by IL-1/Toll receptors, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M. IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-betaB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.
Background Reference 1:
Cao Z, Henzel WJ, and Gao X. IRAK: a kinase associated with the interleukin-1 receptor. Science 1996; 271:1128-31.
Background Reference 2:
Muzio M, Ni J, Feng P, et al. IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling. Science 1997; 278:1612-5.
Background Reference 3:
Wesche H, Gao X, Li X, et al. IRAK-M is a novel member of the Pelle/interleukin-1 receptor-associated kinase (IRAK) family. J. Biol. Chem. 1999; 274:19403-10.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2355

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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