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45-778 (0.05μg/ml) staining of Rat Liver lysate (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected with chemiluminescence.
45-778 (0.5μg/ml) staining of Mouse Bone Marrow (wildtype first lane, KO second lane) lysates (35μg protein in RIPA buffer). Primary incubation was overnight at 4C Detected with chemiluminescence. Data obtained from anonymous customer
45-778 (0.05μg/ml) staining of Rat Liver lysate (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected with chemiluminescence.

IRAK3 Antibody

CATALOG NUMBER: 45-778

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-GHSYGSKPMEKR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65kDa band observed in Rat Liver lysates (calculated MW of 67.8kDa according to NP_082955.2). An approx 70kDa is observed in Mouse Bone Marrow lysates but this band disappears in lysates from the knock-out mouse. Recommended concentration: 0.05-0.2ug/ml.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
IRAK3
Additional Names:
IRAK3, interleukin-1 receptor-associated kinase 3, ASRT5, IRAK-M, IRAKM, interleukin-1 receptor-associated kinase M
Protein Accession Number:
NP_082955.2
Protein Gi Number:
31543005
Ncbi Gene Id Number:
73914(mouse);314870(rat);
Background Reference 1:
Balaci L, Spada MC, Olla N, Sole G, Loddo L, Anedda F, Naitza S, Zuncheddu MA, Maschio A, Altea D, Uda M, Pilia S, Sanna S, Masala M, Crisponi L, Fattori M, Devoto M, Doratiotto S, Rassu S, Mereu S, Giua E, Cadeddu NG, Atzeni R, Pelosi U, Corrias A, Perra R., Torrazza PL, Pirina P, Ginesu F, Marcias S, Schintu MG, Del Giacco GS, Manconi PE, Malerba G, Bisognin A, Trabetti E, Boner A, Pescollderungg L, Pignatti PF, Schlessinger D, Cao A, Pilia G. IRAK-M is involved in the pathogenesis of early-onset persistent asthma. Am J Hum Genet. 2007 Jun;80(6):1103-14

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-778

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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