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Western blot analysis of extracts of various cell lines, using IRAK4 antibody (24-025) at 1:1000 dilution.<br />Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br />Lysates/proteins: 25ug per lane.<br />Blocking buffer: 3% nonfat dry milk in TBST.<br />Detection: ECL Basic Kit.<br />Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using IRAK4 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using IRAK4 antibody at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using IRAK4 antibody (24-025) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

IRAK4 Antibody

CATALOG NUMBER: 24-025

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human IRAK4 (NP_057207.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
Jurkat
Positive Control 3:
Raji
Positive Control 4:
U-251MG
Predicted Molecular Weight:
Observed: 55 kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IRAK4
Additional Names:
IRAK-4 Antibody: IPD1, REN64, IRAK-4, NY-REN-64, Interleukin-1 receptor-associated kinase 4, Renal carcinoma antigen NY-REN-64
Ncbi Gene Id Number:
51135
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

24-025

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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