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Western blot analysis of IRE1p in Raji cell lysate with IRE1p antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of IRE1p in Raji cell lysate with IRE1p antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

IRE1p Antibody

CATALOG NUMBER: 3659

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (80%)
Immunogen:
IRE1p antibody was raised against a 15 amino acid synthetic peptide from near the center of human IRE1p.
The immunogen is located within amino acids 800 - 850 of IRE1p.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
IRE1p antibody can be used for the detection of IRE1p by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1207 - Raji Cell Lysate
Purification:
IRE1p Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IRE1p Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IRE1p antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ERN1
Additional Names:
IRE1p Antibody: IRE1, IRE1P, IRE1a, hIRE1p, IRE1, Endoplasmic reticulum-to-nucleus signaling 1
Protein Accession Number:
O75460
Protein Gi Number:
193806335
Ncbi Gene Id Number:
2081
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRE1p Antibody: Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94. These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background Reference 1:
Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr.1994; 4:1-18.
Background Reference 2:
Lee AS. The ER chaperone and signaling regulator GRP78/BiP as a monitor of endoplasmic reticulum stress. Methods2005; 35:373-81.
Background Reference 3:
Bertolotti A, Zhang Y, Hendershot LM, et al. Dynamic interaction of BiP and ER stress transducers in the unfolded-protein response. Nat. Cell Biol.2000; 2:326-32.
Background Reference 4:
Shen J, Chen X, Hendershot L, et al. ER stress regulation of ATF6 localization by dissociation of BiP/GRP78 binding and unmasking of Golgi localization signals. Dev. Cell2002; 3:99-111.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3659

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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