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42-103 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-103 (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-103 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

IREB2 Antibody

CATALOG NUMBER: 42-103

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat
Immunogen:
The immunogen for this antibody is: C-SIHYEGSEYKLSHGS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Human Liver lysates (calculated MW of 105kDa according to NP_004127.1). Recommended concentration: 1-3ug/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was confirmed as a breakdown product in aged purified recombinant protein by a free sample recipient.Immunohistochemistry:In paraffin embedded Human Kidney shows preferential staining in the cytoplasm of DCT. Recommended concentration, 3-6ug/ml.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
IREB2
Additional Names:
IREB2, iron-responsive element binding protein 2, ACO3, FLJ23381, IRP2, IRP2AD, iron regulatory protein 2
Protein Accession Number:
NP_004127.1
Protein Gi Number:
41352693
Ncbi Gene Id Number:
3658
Background Reference 1:
Wang W, Di X, D1Agostino RB, Torti SV, Torti FM, Excess capacity of the iron regulatory protein system. The Journal of biological chemistry 2007 Aug 282 (34): 24650-9.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-103

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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