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Western blot analysis of IRF2BP2 in HeLa cell lysate with IRF2BP2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Immunocytochemistry of IRF2BP2 in A20 cells with IRF2BP2 antibody at 2.5 μg/mL.
Western blot analysis of IRF2BP2 in HeLa cell lysate with IRF2BP2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Trial Size

IRF2BP2 Antibody

CATALOG NUMBER: 6705

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
IRF2BP2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human IRF2BP2.
The immunogen is located within amino acids 440 - 490 of IRF2BP2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
IRF2BP2 antibody can be used for detection of IRF2BP2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of IRF2BP2 are known to exist; this antibody will detect both. This antibody is predicted to not cross-react with any FOXD4 protein family members.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-208 - A-20 Cell Slide
Purification:
IRF2BP2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IRF2BP2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IRF2BP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IRF2BP2
Additional Names:
IRF2BP2 Antibody: Interferon regulatory factor 2-binding protein 2, IRF-2-binding protein 2
Protein Accession Number:
NP_892017
Protein Gi Number:
116734704
Ncbi Gene Id Number:
359948
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRF2BP2 Antibody: IRF2BP2, like the related protein IRF2BP1, is a co-repressor that interacts specifically with the C-terminal repression domain of Interferon Regulatory Factor 2 (IRF2). IRF2BP2 is a direct target gene of p53 and is involved in cell survival during the p53 stress response, able to impede the p53-mediated transactivation of p21 and Bax. IRF2BP2 is also a co-factor of VGLL4 and is required to induce the expression of vascular endothelial growth factor A (VEGF-A) in muscle. It is normally found in the nucleus of skeletal muscle and cardiac cells, but can be found in the cytoplasm during skeletal muscle differentiation.
Background Reference 1:
Childs KS and Goodbourn S. Identification of novel co-repressor molecules for interferon regulatory factor-2. Nuc. Acids Res. 2003; 31:3016-26
Background Reference 2:
Koeppel M, van Heeringen SJ, Smeenk L, et al. The novel p53 target gene IRF2BP2 participates in cell survival during the p53 stress response. Nuc. Acids Res. 2009; 37:322-35.
Background Reference 3:
Teng ACT, Kuraitis D, Deeke SA, et al. IRF2BP2 is a skeletal and cardiac muscle-enriched ischemia-inducible activator of VEGFA expression. FASEB J. 2010; 24:4825-34.
Background Reference 4:
Teng ACT, Al-montashiri NAM, Cheng BLM, et al. Identification of a phosphorylation-dependent nuclear localization motif in interferon regulatory factor 2 binding protein 2. PLoS One 2011; 6:e24100.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6705

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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