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Western blot analysis of IRF3 in Ramos whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Immunohistochemical staining of mouse kidney using IRF3 antibody at 2 μg/mL.
Immunofluorescence of IRF3 in Mouse Kidney cells with IRF3 antibody at 20 μg/mL.
Western blot analysis of IRF3 in Ramos whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Trial Size

IRF3 Antibody

CATALOG NUMBER: 3397

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (86%), Pig: (79%)
Immunogen:
IRF3 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human IRF3.
The immunogen is located within amino acids 330 - 380 of IRF3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
IRF3 antibody can be used for detection of IRF3 by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of IRF3 are known to exist.
Positive Control 1:
Cat. No. 1225 - Ramos Cell Lysate
Positive Control 2:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Predicted Molecular Weight:
43, 47, 50 kDa
Purification:
IRF3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IRF3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IRF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IRF3
Additional Names:
IRF3 Antibody: Interferon regulatory factor 3, IRF-3
Protein Accession Number:
NP_001562
Protein Gi Number:
4504725
Ncbi Gene Id Number:
3661
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRF3 Antibody: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF3 is present in an inactive form in the cytoplasm of most cells. Following viral infection, IRF3 can be activated by IκB kinase-ε and TANK-binding kinase 1 (TBK1), whereupon IRF3 translocates to the nucleus. IRF3 can also be activated by stimulation of toll-like receptor 3 (TLR3) by dsRNA. IRF3 exists as at least two distinct isoforms.
Background Reference 1:
Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54
Background Reference 2:
Au WC, Moore PA, Lowther W, et al. Identification of a member of the interferon regulatory factor family that binds to the interferon-stimulated response element and activates expression of interferon-induced genes. Proc. Natl. Acad. Sci. USA 1995; 92:11657-61.
Background Reference 3:
Fitzgerald KA, McWhirter SM, Faia KL, et al. IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway. Nat. Immunol. 2003; 4:491-6.
Background Reference 4:
Sharma S, Tenoever BR, Grandvaux N, et al. Triggering the interferon antiviral response through an IKK-related pathway. Science 2003; 300:1148-51.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3397

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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