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Western blot analysis of extracts of various cell lines, using IRF3 antibody (13-919) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Western blot analysis of extracts from normal (control) and IRF3 knockout (KO) HeLa cells, using IRF3 antibody (13-919) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human lung cancer using IRF3 antibody (13-919) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using IRF3 antibody (13-919) at dilution of 1:100 (40x lens).
Chromatin immunoprecipitation analysis of extracts of HCT116 cell line, using IRF3 rabbit polyclonal antibody (13-919) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western blot analysis of extracts of various cell lines, using IRF3 antibody (13-919) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
KO

IRF3 Antibody, KO Validated

CATALOG NUMBER: 13-919

Clonality:
Polyclonal
Tested Applications:
ChIP, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human IRF3 (NP_001184051.1).
Conjugate:
Unconjugated
Tested Applications:
ChIP, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
ChIP: 1:20 - 1:100
Positive Control 1:
293T
Positive Control 2:
HeLa
Positive Control 3:
Jurkat
Positive Control 4:
HepG2
Predicted Molecular Weight:
Observed: 56kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IRF3
Additional Names:
IRF3 Antibody: Interferon regulatory factor 3, IRF-3
Ncbi Gene Id Number:
3661
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-919

List Size:
100 uL

List Price:

$436.00

Datasheet
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