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45-075 staining (1ug/ml) Human Spleen (A) and Peripheral Blood Lymphocytes (B) lysates (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence
45-075 staining (1ug/ml) Human Spleen (A) and Peripheral Blood Lymphocytes (B) lysates (RIPA buffer, 30ug total protein per lane). Detected by chemiluminescence

IRF5 Antibody

CATALOG NUMBER: 45-075

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Cow
Immunogen:
The immunogen for this antibody is: C-QGPWPMHPAGMQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 60kDa band observed in Human Spleen and Peripheral Blood Lymphocytes lysates.The calculated molecular size is 57.8kDa according to NP_001092099.1. Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:This antibody showed nuclear staining in Human Cervix, as confirmed by customer (data not shown). This data is from a previous batch, not on sale.Chromatin immunoprecipitation: This antibody has been successfully used in human monocyte-derived dendritic cells.This data is from a previous batch, not on sale.
Specificity:
This antibody is expected to recognize all four reported isoforms (NP_002191.1; NP_116032.1; NP_001092097.1; NP_001092099.1). Reported variants NP_001092100.1, NP_001092101.1, NP_001092098.1, NP_001092097.1 represent identical protein: Reported variants NP_116032.1, NP_001092100.1 represent identical protein:
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
IRF5
Additional Names:
IRF5, interferon regulatory factor 5, SLEB10
Protein Accession Number:
NP_002191.1,NP_116032.1,NP_001092097.1,NP_001092099.1
Protein Gi Number:
4504727
Ncbi Gene Id Number:
3663
Background Reference 1:
Barnes BJ, Moore PA, Pitha PM. Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes. J Biol Chem. 2001 Jun 29;276(26):23382-90.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-075

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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