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<strong>Figure 1 KO Validation in A549 Cell Lysate</strong><br> Loading: 10 μg of A549 WT cell lysates or IRF7 KO cell lysates. Antibodies: IRF7 8991 (0.5 μg/mL) and beta-actin 3779 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IRF7, 8991, (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Human Tissues</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IRF7, 8991 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Mouse Tissues</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IRF7, 8991 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Western Blot Validation in Rat Tissues</strong><br>Loading: 15 μg of lysates per lane.Antibodies: IRF7, 8991 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 6 Immunofluorescence Validation of IRF7 in Mouse Raw264.7 Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed Raw264.7 cells labeling IRF7 with 8991 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 7 Immunohistochemistry Validation of IRF7 in Human Colon Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-IRF7 antibody (8991) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 8 Immunohistochemistry Validation of IRF7 in Human Pancreas Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IRF7 antibody (8991) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 9 Immunohistochemistry Validation of IRF7 in Human Liver Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IRF7 antibody (8991) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 10 Immunohistochemistry Validation of IRF7 in Mouse Pancreas Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-IRF7 antibody (8991) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 11 Immunohistochemistry Validation of IRF7 in Rat Brain Tissue</strong><br> Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-IRF7 antibody (8991) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 KO Validation in A549 Cell Lysate
Loading: 10 μg of A549 WT cell lysates or IRF7 KO cell lysates. Antibodies: IRF7 8991 (0.5 μg/mL) and beta-actin 3779 (1 µg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
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IRF7 Antibody

CATALOG NUMBER: 8991

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti-IRF7 antibody (8991) was raised against a peptide corresponding to 17 amino acids near the center of human IRF7.
The immunogen is located within amino acids 200-230 of IRF7.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WB: 0.5-1 μg/mL; IF: 20 μg/mL; IHC: 2-5 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples. Immunofluorescence in mouse samples. Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
Specificity:
Several isoforms of IRF7 are known to exist.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 2:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 3:
Cat. No. 1211 - HepG2 Cell Lysate
Validation:

KO Validation (Figure 1): Anti-IRF7 antibodies (8991) specificity was further verified by IRF7 specific knockout. IRF7 was disrupted in IRF7 knockout A549 cells as compared to that in control wild type cells.

Isoforms:
Human IRF7 has 4 isoforms, including isoform A (503aa, 54kD), isoform B (474aa, 52kD), isoform C (164aa, 18kD) and isoform D (516aa, 56kD). Mouse IRF7 has one isoform (457aa, 51kD) and Rat IRF7 also has one isoform (456aa, 51kD). 8991 can detect human isoforms and mouse, and possibly can detect rat.
Purification:
IRF7 Antibody is Protein A purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IRF7 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IRF7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IRF7
Additional Names:
IRF7 Antibody: IRF7A, IRF7B, IRF7C, IRF7H, IRF-7H, Interferon regulatory factor 7, IRF-7
Protein Accession Number:
Q92985
Protein Gi Number:
116242593
Ncbi Gene Id Number:
3665
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRF7 Antibody: Interferons (IFNs) are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. IRF7 play a major role in the innate immune pathway, interacting with the Toll-like receptor (TLR) adaptor proteins MyD88 and Tirp/TRAM and functioning as an intermediate TLR4 and TLR9 signaling. There are at least four differentially spliced isoforms of IRF7, although their function has not been clearly established.
Background Reference 1:
Malmgaard. J. Interferon & Cyto. Res. 2004; 24:439-54.
Background Reference 2:
Sato et al. Immunity 2000; 13:539-48.
Background Reference 3:
Fitzgerald et al. J. Exp. Med. 2003; 198:1043-55.
Background Reference 4:
Honda et al. Proc. Natl. Acad. Sci. USA 2004; 101:15416-21.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8991

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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