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Western blot analysis of IRF8 in human thymus tissue lysate with IRF8 antibody at 1μg/mL in (A) the presence and (B) absence of blocking peptide.
Immunofluorescence of IRF8 in K562 cells with IRF8 antibody at 10 μg/mL.
Western blot analysis of IRF8 in human thymus tissue lysate with IRF8 antibody at 1μg/mL in (A) the presence and (B) absence of blocking peptide.
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IRF8 Antibody

CATALOG NUMBER: 3401

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IRF8 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRF8.
The immunogen is located within the last 50 amino acids of IRF8.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
IRF8 antibody can be used for detection of IRF8 by Western blot at 1 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1314 - Human Thymus Tissue Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Purification:
IRF8 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
IRF8 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IRF8 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IRF8
Additional Names:
IRF8 Antibody: Interferon Regulatory Factor, Interferon Consensus Sequence Binding Protein, H-ICSBP, ICSBP1, ICSBP, IRF-8, Interferon Consensus Sequence-Binding Protein, IMD32A, IMD32B
Protein Accession Number:
Q02556
Ncbi Gene Id Number:
3394
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRF8 Antibody: Interferons (IFN)s are involved in a multitude of immune interactions during viral infections and play a major role in both the induction and regulation of innate and adaptive antiviral mechanisms. During infection, host-virus interactions signal downstream molecules such as transcription factors such as IFN regulatory factor-3 (IRF3) which can act to stimulate transcription of IFN-alpha/beta genes. Unlike IRF3, IRF8 appears to act as a negative regulator of IFN-induced genes in most cases, but IRF8 mediates activation of NF-κB by the toll-like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells. Finally, it has been shown that IRF8 decreases bcl-2 expression and thus may play a role in chronic myelogenous leukemia.
Background Reference 1:
Malmgaard L. Induction and regulation of IFNs during viral infections. J. Interferon & Cyto. Res. 2004; 24:439-54
Background Reference 2:
Weisz A, Marx P, Sharf R, et al. The human interferon consensus sequence binding protein (H-ICSBP) is a negative regulator of enhancer elements common to interferon inducible genes. J. Biol. Chem. 1992; 267:25589-96.
Background Reference 3:
Nelson N, Marks MS, Driggers PH, et al. Interferon consensus sequence-binding protein, a member of the interferon regulatory factor family, suppresses interferon-induced gene transcription. Mol. Cell. Biol. 1993; 13:588-99.
Background Reference 4:
Tsujimura H, Tamura T, Kong HJ, et al. Toll-like receptor 9 signaling activates NF-κB through IFN regulatory factor-8/IFN consensus sequence binding protein in dendritic cells. J. Immunol. 2004; 172:6820-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3401

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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