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Western Blot at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
IRF9 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of IRF9 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Western Blot at 1:2000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.

IRF9 Antibody

CATALOG NUMBER: 56-092

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
This IRF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-104 amino acids from the N-terminal region of human IRF9.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
44 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IRF9
Additional Names:
Interferon regulatory factor 9, IRF-9, IFN-alpha-responsive transcription factor subunit, ISGF3 p48 subunit, Interferon-stimulated gene factor 3 gamma, ISGF-3 gamma, Transcriptional regulator ISGF3 subunit gamma, IRF9, ISGF3G
Protein Accession Number:
Q00978
Protein Gi Number:
266392
Ncbi Gene Id Number:
10379
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRF9 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Background Reference 1:
Maiwald, T., et al. FEBS J. 277(22):4741-4754(2010)
Background Reference 2:
Schuurhof, A., et al. Pediatr. Pulmonol. 45(6):608-613(2010)
Background Reference 3:
Watanabe, T., et al. J. Clin. Invest. 120(5):1645-1662(2010)
Background Reference 4:
Mosbruger, T.L., et al. J. Infect. Dis. 201(9):1371-1380(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-092

List Size:
400 ul

List Price:

$495.00

Datasheet
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