Western blot analysis of extracts from normal (control) and IRF9 knockout (KO) A-549 cells, using IRF9 antibody (18-195) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

Western blot analysis of extracts from normal (control) and IRF9 knockout (KO) A-549 cells, using IRF9 antibody (18-195) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

IRF9 Antibody, KO Validated

CATALOG NUMBER: 18-195

Clonality:

Polyclonal

Tested Applications:

Host Species:

Rabbit

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 113-255 of human IRF9 (NP_006075.3).

Conjugate:

Unconjugated

Tested Applications:

Application Note:

WB: 1:500 - 1:2000

Positive Control 1:

A-549

Predicted Molecular Weight:

Observed: 43kDa

Advanced Validation

Validation:

Antibody is Knockout validated.

Properties

Purification:

Affinity purification

Clonality:

Polyclonal

Isotype:

IgG

Physical State:

Liquid

Buffer:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration:

batch dependent

Storage Conditions:

Store at -20°C. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

IRF9

Additional Names:

ISGF3G, IRF-9, ISGF3, ISGF3G, p48

Ncbi Gene Id Number:

10379

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-195

List Size:

50 uL

List Price:

$369.00

Out of stock

Datasheet

Shipping Info

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IRF9 Antibody, KO Validated

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Predicted Molecular Weight:

Validation:

Purification:

Clonality:

Isotype:

Physical State:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Disclaimer:

List Size:

Shipping Info

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