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Western blot analysis of IRGC in mouse brain tissue lysate with IRGC antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of IRGC in A20 cells with IRGC antibody at 2.5 μg/mL.
Immunofluorescence of IRGCb in A20 cells with IRGCb antibody at 20 μg/mL.
Western blot analysis of IRGC in mouse brain tissue lysate with IRGC antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

IRGC Antibody

CATALOG NUMBER: 5295

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IRGC antibody was raised against a 12 amino acid synthetic peptide near the carboxy terminus of human IRGC.
The immunogen is located within the last 50 amino acids of IRGC.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
IRGC antibody can be used for detection of IRGC by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2:
Cat. No. 17-208 - A-20 Cell Slide
Purification:
IRGC Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IRGC Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IRGC antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IRGC
Additional Names:
IRGC Antibody: IFGGE, IRGC1, Iigp5, CINEMA, R30953_1, IIGP5, Interferon-inducible GTPase 5, Immunity-related GTPase cinema 1
Protein Accession Number:
NP_062558
Protein Gi Number:
10257429
Ncbi Gene Id Number:
56269
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRGC Antibody: Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans.
Background Reference 1:
Taylor GA. IRG proteins: key mediators of interferon-regulated host resistance to intracellular pathogens. Cell Microbiol.2007; 9:1099-107.
Background Reference 2:
Yamada K, Akimoto H, Ogawa Y, et al. Upregulation of immunity-related GTPase (IRG) proteins by TNF-alpha in murine astrocytes. Biochem. Biophys. Res. Commun.2009; 382:434-9.
Background Reference 3:
Singh SB, et al. Human IRGM induces autophagy to eliminate intracellular mycobacteria. Science2006; 313:1438-41.
Background Reference 4:
Bekpen C, Hunn JP, Rohde C, et al. The interferon-inducible p47 (IRG) GTPases in vertebrates: loss of the cell autonomous resistance mechanism in the human lineage. Genome Biol.2005; 6:R92.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5295

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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