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Western blot analysis of IRGM expression in SK-N-SH cell lysate with IRGM antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of CD4 in human thymus tissue with CD4 antibody at 5 μg/ml.
Immunofluorescence of IRGM in Human Brain tissue with IRGM antibody at 20 μg/mL.<br><br>Green: IRGM Antibody (4543) <br> Blue: DAPI staining
Western blot analysis of IRGM expression in SK-N-SH cell lysate with IRGM antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

IRGM Antibody

CATALOG NUMBER: 4543

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IRGM antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of the human IRGM.
The immunogen is located within amino acids 30 - 80 of IRGM.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
IRGM antibody can be used for detection of IRGM by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1220 – SK-N-SH Cell Lysate
Predicted Molecular Weight:
Predicted: 20 kDa
Observed: 20 kDa 
Purification:
IRGM Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IRGM Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IRGM antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
IRGM
Additional Names:
IRGM Antibody: IFI1, IRGM1, LRG47, LRG-47, IFI1, Immunity-related GTPase family M protein, Immunity-related GTPase family M protein 1
Protein Accession Number:
AAI28169
Protein Gi Number:
118764009
Ncbi Gene Id Number:
345611
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
IRGM Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Two of the strongest hits implicate genes IRGM and ATG16L1, which encode proteins thought to be critical to the autophagy pathway and being significantly associated with Crohn's disease. IRGM induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. In mouse, IRGM belongs to a family of gamma-interferon-induced GTP-binding proteins of approximately 48 kDa that also includes IRGM2 and IRGM3; this antibody may also recognize these proteins.
Background Reference 1:
Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906.
Background Reference 2:
Massey DC and Parkes M. Genome-wide association scanning highlights two autophagy genes, ATG16L1 and IRGM, as being significantly associated with Crohn's disease. Autophagy2007; 3:649-51.
Background Reference 3:
Fisher SA, Tremelling M, Anderson CA, et al. Genetic determinants of ulcerative colitis include the ECM1 locus and five loci implicated in Crohn's disease. Nat. Genet.2008; 40:710-2.
Background Reference 4:
Singh SB, Davis AS, Taylor GA, et al. Human IRGM induces autophagy to eliminate intracellular mycobacteria. Science2006; 313:1438-41.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4543

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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