Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of extract from 293 cells untreated or treated with insulin using IRS-1 (Phospho-Ser312).
Western blot analysis of extract from 293 cells untreated or treated with insulin using IRS-1 (Phospho-Ser312).

IRS 1 (phospho Ser312) Antibody

CATALOG NUMBER: 79-458

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
IRS-1 (Phospho-Ser312) antibody was raised against a peptide sequence around phosphorylation site of serine 312 (A-T-S (p) -P-A) derived from Human IRS-1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
This antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 312.
Predicted Molecular Weight:
180 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
IRS1
Additional Names:
HIRS-1, OCT1, OTF1, HIRS-1, IRS1, Insulin receptor substrate 1
Protein Accession Number:
NP_005535.1 
Protein Gi Number:
5031805
Ncbi Gene Id Number:
3667
Background:
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit
Background Reference 1:
Tzatsos A, et al. (2006) Mol Cell Biol; 26 (1): 63-76Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98 (8): 4640-4645Szanto I, et al. (2000) Proc Natl Acad Sci U S A; 97 (5): 2355-2360Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98 (8): 4640-4645

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-458

List Size:
0.1 mg

List Price:

$376.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369