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43-180 (0.2ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-180 (0.2ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Irs4 Antibody

CATALOG NUMBER: 43-180

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-DRGTKERKEAKEVRD
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Mouse Heart lysates (calculated MW of 130kDa according to NP_034702.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreyer et al, jEndocrinology. 2003 Apr;144(4):1211-8. PMID: 12639902). Recommended concentration: 0.2-0.6ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at ~39kDa. This band was successfully blocked by incubation with the immunizing peptide. This product has been successfully used in WB on Mouse: Ikink GJ et al, Nat Commun. 2016 Nov 23;7:13567. PMID: 27876799.Immunofluorescence: This product has been successfully used in IF on Mouse: Harriet M Jackson et al, BMC Genomics. 2013 Nov 25;14:831. PMID: 24274089.
Predicted Molecular Weight:
Approx 150kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Irs4
Additional Names:
Irs4, insulin receptor substrate 4, IRS-4, RP23-415G1.3, phosphoprotein of 160 kDa, pp160
Protein Accession Number:
NP_034702.2
Protein Gi Number:
58331138
Ncbi Gene Id Number:
16370
Background Reference 1:
Melkersson K, Persson B.Association between body mass index and insulin receptor substrate-4 (IRS-4) gene polymorphisms in patients with schizophrenia.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-180

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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